Refractive index-assisted UV/Vis spectrophotometry to overcome spectral interference by impurities

A major challenge hindering the application of techniques like UV/Vis spectrophotometry in determining concentration is spectral interference from contaminants. Since molar absorptivities vary significantly, even minuscule amounts of specific contaminants may cause relatively large errors in UV/Vis spectrophotometry based quantification. Current methods to deal with this are slow, cost-intensive, or ineffective for unknown interferents. We propose constrained refractometry as an expedient technique to aid UV/Vis spectrophotometry, avoiding large errors due to spectral interference. Based on a modified Lorentz Lorenz equation, the technique helps not only in detecting and reducing error from unknown contaminants but also in identifying the significant impurity. Experimental results show a significant reduction of error in concentration determination even for multiple unknown interfering contaminants.