Evaluation of tissue inflammatory response of four intracanal medicament - An animal study

The aim of the study was to evaluate the tissue inflammatory response of origanum vulgarae, omeprazole, triple antibiotic paste (TAP), and calcium hydroxide in an animal model.

Seventy Five male Wistar rats were randomly divided into four experimental groups and one control group: Group 1: control (n = 15), Group 2: 0.25 mg origanum vulgarae/1 ml of cellulose (n = 15), Group 3: 2 mg omeprazole/1 ml of distilled water (n = 15), and Group 4: 1 mg TAP/1 ml of distilled water (n = 15), Group 5: 16 mg calcium hydroxide/1 ml of distilled water (n = 15). A trough was made in the periapical bone and the medicament of the respective groups was placed. After the 7^th, 14^th, 28^th and days, the animals were euthanized and tissue specimen was prepared for histological examination.

The results were analyzed statistically. On the 7^th and 14^th days, all the experimental groups showed severe inflammatory response with no statistical significance, whereas on the 28^th day, the inflammatory response was graded based on the mean value, in which omeprazole showed moderate inflammatory cells followed by TAP. Mild inflammatory response was seen in calcium hydroxide and origanum vulgarae, showing no statistical significance.

Within the limitation of the study, severe inflammatory response was reported on the 7^th and 14^th days in all experimental groups, whereas on 28^th day, there was a moderate inflammatory response seen in omeprazole, followed by TAP. Calcium hydroxide and origanum vulgarae showed a mild inflammatory response.