Efficacy of hydroxyapatite and silica nanoparticles on erosive lesions remineralization

The aim is to assess and compare the mineral gain and penetration depth of hydroxyapatite and silica nanoparticle infiltrates into artificially created erosive lesions of enamel and dentin.

Sixty extracted human molars were sectioned to obtain enamel and dentin samples (n = 60 each). They were demineralized using citric and formic acid, respectively, to create erosive lesions on enamel and dentin surfaces. Samples were assigned into nanohydroxyapatite (nHA) or nanosilica groups (n = 30 each) according to the infiltrant used. Half of the enamel and dentin samples in each group (n = 15) were analyzed after erosive attack for mineral loss, after infiltrant application for mineral gain, using energy-dispersive X-ray spectroscopy. In another half of the enamel and dentin samples (n = 15), the penetration depth of the nanoinfiltrants was analyzed using confocal microscopy.

To compare the overall mineral gain between groups, a dependent t-test was applied. The intergroup comparisons were made using one-way ANOVA followed by Tukey post hoc test for pairwise comparisons for both penetration depth and mineral gain. The significance level was set to P ≤ 0.05.

The mineral gain in enamel was not statistically different between nHA and nanosilica infiltrants (P = 0.9950). nHA infiltrated dentin showed significantly more mineral gain (P = 0.0001) than nanosilica infiltrant. The depth of penetration of the nHA in enamel was statistically greater than that of nanosilica, but in dentin, the difference was not significant.

nHA infiltrant performed better in mineral gain, and penetrated deeper into the demineralized erosive lesions, compared to nanosilica infiltrant in both enamel and dentin. The highest mineral precipitation and deeper penetration into both demineralized enamel and dentin was observed with nHA infiltrant compared to (as against) nanosilica infiltrant.