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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Nov 18, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology.

Yan Qiu¹, Qiurong Ding^1,2

¹CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, P. R. China.

|February 8, 2021

Summary

This summary is machine-generated.

This study details a CRISPR-Cas gene editing protocol for adipocytes. It covers vector preparation, lentivirus transduction, and dual-gene editing for enhanced research applications.

Keywords:

CRISPR Cell culture Metabolism Molecular biology

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Last Updated: Nov 18, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

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Published on: May 25, 2018

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Gene editing technologies are crucial for understanding cellular functions.
Adipocytes play key roles in metabolism and disease.
Efficient gene editing in adipocytes is essential for functional studies.

Purpose of the Study:

To provide a comprehensive protocol for CRISPR-Cas gene editing in adipocytes.
To describe methods for sgRNA design, vector preparation, and lentivirus transduction.
To present an optimized dual-gene editing strategy in adipocytes.

Main Methods:

CRISPR-Cas gene editing protocol development.
Single and dual sgRNA design and vector construction (lentiCRISPRv2).
Lentivirus preparation and transduction of adipocytes.
Functional validation of sgRNAs.

Main Results:

A detailed and reproducible protocol for gene editing in adipocytes was established.
Successful lentivirus transduction and gene editing in adipocytes demonstrated.
An optimized method for targeting two distinct genes simultaneously in adipocytes was described.

Conclusions:

The presented protocol enables efficient CRISPR-Cas mediated gene editing in adipocytes.
This methodology facilitates functional genomic studies in adipocyte biology.
The dual-gene editing approach offers advanced tools for complex genetic investigations in adipocytes.