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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Nov 18, 2025

Flow Cytometric Characterization of Murine B Cell Development
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Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

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Flow Cytometric Characterization of Murine B Cell Development.

Faith M Harris1, Karoline A Meagher2, Maggie Zhong1

  • 1Regeneron Tech Centers, Regeneron Pharmaceuticals, Inc.

Journal of Visualized Experiments : Jove
|February 8, 2021
PubMed
Summary
This summary is machine-generated.

Flow cytometry is essential for evaluating murine B cell development in various organs. Adhering to best practices ensures accurate data for therapeutic antibody development in autoimmune and humanized mouse models.

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Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Murine B cell development and differentiation are extensively studied in secondary lymphoid organs.
  • Antibodies produced by B cells are key components of established therapeutics.
  • Understanding B cell development is critical for evaluating immunomodulatory drugs and antibody-based therapies.

Purpose of the Study:

  • To outline best practices for using flow cytometry to assess murine B cell development.
  • To highlight the importance of flow cytometry in validating therapeutic agents in preclinical models.
  • To emphasize the complementary nature of flow cytometry with other B cell assessment methods.

Main Methods:

  • Utilizing established B cell flow cytometric parameters to analyze B cell populations.
  • Evaluating B cell development across multiple murine compartments: peritoneum, bone marrow, and spleen.
  • Integrating flow cytometric data with additional immunological readouts.

Main Results:

  • Established flow cytometric parameters effectively characterize B cell development in murine models.
  • Adherence to specific best practices is crucial for reliable and reproducible results.
  • Flow cytometry provides valuable insights into B cell dynamics in wild-type, autoimmune-prone, and humanized mice.

Conclusions:

  • Flow cytometry is a validated and critical tool for assessing B cell development in mice.
  • Standardized flow cytometric analysis supports the preclinical development of antibody therapeutics.
  • This methodology enhances the understanding of B cell biology in diverse mouse models relevant to human diseases and therapy.