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Updated: Nov 18, 2025

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins
Taishi Kondo1, Minori Eguchi, Seita Kito
1Graduate School of Engineering, Nagoya University, Nagoya, 464-8603, Japan. murah@chembio.nagoya-u.ac.jp.
Abstract:
We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.
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