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Related Experiment Video

Updated: Nov 18, 2025

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
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Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

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TAEL 2.0: An Improved Optogenetic Expression System for Zebrafish.

Jesselynn LaBelle1, Adela Ramos-Martinez1, Kyle Shen1

  • 1Department of Molecular Cell Biology, University of California Merced, Merced, California, USA.

Zebrafish
|February 8, 2021
PubMed
Summary
This summary is machine-generated.

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Quantification of <i>eef1a1l1</i> transcript in early zebrafish development.

microPublication biology·2026

Researchers enhanced the TAEL optogenetic gene expression system for zebrafish, creating TAEL 2.0. This improved system offers faster, higher gene induction with applications in late-stage zebrafish development.

Area of Science:

  • Optogenetics
  • Gene expression regulation
  • Zebrafish developmental biology

Background:

  • Inducible gene expression systems are crucial for biological research.
  • The original TAEL system provided light-inducible gene expression in zebrafish.
  • Limitations in temporal and spatial control and late-stage induction existed.

Purpose of the Study:

  • To improve the TAEL optogenetic gene expression system for enhanced performance in zebrafish.
  • To develop TAEL 2.0 with modifications to the transcriptional activator and promoter.
  • To create stable transgenic zebrafish lines expressing the improved system.

Main Methods:

  • Modifying the TAEL transcriptional activator and C120 regulatory element.
  • Assessing reporter gene expression levels and kinetics.
Keywords:
gene expressionlight-activated transcription factoroptogeneticszebrafish

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  • Generating and testing stable transgenic zebrafish lines with ubiquitous and tissue-specific TAEL 2.0 expression.

    • Main Results:

    • TAEL 2.0 demonstrated significantly higher and faster reporter gene induction compared to the original TAEL.
    • Background expression and toxicity remained comparable to the original system.
    • Functional stable transgenic lines were successfully created, including a ubiquitous line effective in late embryonic and larval stages.

    Conclusions:

    • TAEL 2.0 represents a significant advancement in optogenetic gene expression for zebrafish.
    • The enhanced system provides superior spatial and temporal control and overcomes previous limitations in late-stage induction.
    • TAEL 2.0 will be a valuable tool for the zebrafish research community.