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Related Concept Videos

DNA Replication02:40

DNA Replication

55.5K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Overview
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
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Related Experiment Video

Updated: Nov 18, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

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Mammalian DNA Replication Timing.

Athanasios E Vouzas1, David M Gilbert1

  • 1Department of Biological Science, Florida State University, Tallahassee, Florida 32306, USA.

Cold Spring Harbor Perspectives in Biology
|February 9, 2021
PubMed
Summary
This summary is machine-generated.

DNA replication timing and location during the cell cycle are linked to chromatin organization. Recent advances in genomics and imaging illuminate these mechanisms and their relationship to chromosome structure.

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication is semi-conservative and occurs during the S phase of the cell cycle.
  • DNA replication is organized temporally and spatially, linked to chromatin organization.
  • Limited progress in understanding replication regulation has been made until recently.

Purpose of the Study:

  • To review recent discoveries in the temporal and spatial regulation of DNA replication.
  • To explore the causal relationships between DNA replication and chromosome structure/function.
  • To discuss future expectations in the field.

Main Methods:

  • Genomics
  • Genome engineering
  • Advanced imaging techniques

Main Results:

  • Technological advances have enabled detailed study of DNA replication regulation.
  • New insights into the coordination of replication with chromatin structure.
  • Understanding of the interplay between replication timing and nuclear organization.

Conclusions:

  • The field is poised to elucidate mechanisms of DNA replication regulation.
  • Future research will further clarify the links between replication, chromosome structure, and function.
  • Expectations for significant progress in understanding DNA replication in the coming decade.