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Related Experiment Video

Updated: Nov 18, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

Engineering 3D genome organization.

Haifeng Wang¹, Mengting Han¹, Lei S Qi^2,3,4

¹Department of Bioengineering, Stanford University, Stanford, CA, USA.

Nature Reviews. Genetics

|February 9, 2021

Summary

This summary is machine-generated.

Alterations in the 3D genome architecture are linked to diseases. New CRISPR-Cas technologies enable precise manipulation of the 3D genome, advancing our understanding of its role in gene regulation and cellular function.

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

The three-dimensional (3D) genome organization is crucial for regulating gene expression and cellular functions.
Alterations in 3D genome architecture are associated with cancers and developmental disorders.
Understanding the spatio-temporal dynamics of the genome is essential for deciphering its role in health and disease.

Purpose of the Study:

To review recent advances in mammalian 3D genome engineering.
To highlight the application of CRISPR-Cas technologies in manipulating 3D genome organization.
To explore the causal relationship between genome function and its spatio-temporal organization.

Main Methods:

Review of recent literature on 3D genome engineering technologies.
Focus on CRISPR-Cas and related programmable gene-editing tools.
Analysis of technologies enabling manipulation of specific genomic loci.

Main Results:

CRISPR-Cas technologies offer unprecedented capabilities for engineering the 3D genome.
These tools allow precise, programmable manipulation of genome organization.
Advances in 3D genome engineering are rapidly emerging.

Conclusions:

CRISPR-Cas based technologies are revolutionizing the field of 3D genome engineering.
These tools provide powerful means to investigate the functional impact of genome architecture.
Further research in 3D genome engineering holds promise for understanding and treating diseases.