[Study of lymphoid tissue cells by the method of optico-structural machine analysis]

The possibility to use the method of optically-structural machine analysis for automatical classification of lymphoid cells of tumour-bearing rats and lymphoid cells of malignant tumour was studied. The preparations were fixed by methanol, stained by azureosin and scanned on the automatical microscope-analysor "Protva-3". The isolated cells of 6 different groups were scanned. Histogramms of optical density distribution amplitudes were received for every cell and the phase analysis was carried out. The statistical characterization for every cell was based on the results of this analysis. The average values were calculated for 1) the area of the nucleus and cytoplasm: 2) the average density of nucleus and cytoplasm; 3) dispersion of densities of nucleus and cytoplasm; 4) nucleus-cytoplasm relation for every six groups. It was shown that every group of cells may be separated from each other, at least, on the basis of one of the measured parameters. The results are consistent with the morphological data.