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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.

Haixiang Yu¹, Obtin Alkhamis¹, Juan Canoura¹

¹Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL, 33199, USA.

Angewandte Chemie (International Ed. in English)

|February 9, 2021

Summary

Developing aptamer-based biosensors for small molecules is challenging but advancing. This review details aptamer selection, characterization, and sensor development for improved small-molecule detection.

Keywords:

DNA SELEX aptamers biosensors

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Area of Science:

Biochemistry
Molecular Biology
Biosensor Technology

Background:

Aptamers, short oligonucleotides, offer advantages over antibodies as biorecognition elements in biosensors.
Developing aptamer-based sensors, particularly for small molecules, presents significant challenges and is labor-intensive.

Purpose of the Study:

To review challenges and advances in isolating and characterizing small-molecule-binding DNA aptamers.
To discuss the application of aptamers in small-molecule sensing platforms and detection.

Main Methods:

In vitro methodologies for aptamer isolation and selection strategies.
Techniques for aptamer-target binding and structural characterization.
Overview of various small-molecule sensing platforms utilizing aptamers.

Main Results:

Guidance provided on selecting appropriate aptamer generation strategies for optimal binding.
Examination of diverse aptamer-based sensing platforms and their detection applications.
Discussion of engineered aptamers for enhanced sensing capabilities.

Conclusions:

A comprehensive workflow for developing aptamer-based small-molecule sensors for real-world applications is presented.
Advances in aptamer isolation and characterization are crucial for overcoming current limitations.
Aptamers hold significant promise for sensitive and specific small-molecule detection in biosensors.