Molecular characterization of a novel partitivirus isolated from the phytopathogenic fungus Aplosporella javeedii

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Xinru Wan1, Ying Zhao1, Yuanyuan Zhang1

  • 1College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

Archives of Virology
|February 9, 2021
PubMed

Insights

A new mycovirus, Aplosporella javeedii partitivirus 1 (AjPV1), was discovered in a jujube-pathogenic fungus. This finding represents the first mycovirus identified in Aplosporella javeedii.

Area of Science:

  • Mycology
  • Virology
  • Plant Pathology

Background:

  • Aplosporella javeedii is a fungus responsible for canker and dieback diseases in jujube plants, particularly noted in China.
  • Fungal viruses, or mycoviruses, can influence host physiology and pathogenicity, making their discovery crucial for understanding plant diseases.

Purpose of the Study:

  • To identify and characterize novel mycoviruses associated with Aplosporella javeedii.
  • To determine the genetic makeup and phylogenetic relationships of the newly discovered mycovirus.

Main Methods:

  • Isolation of the mycovirus from infected Aplosporella javeedii strain NX55-3.
  • Genome sequencing and analysis of double-stranded RNA (dsRNA) elements.
  • Sequence alignment and phylogenetic analysis to determine viral classification.

Main Results:

  • A new mycovirus, named Aplosporella javeedii partitivirus 1 (AjPV1), was successfully isolated.
  • The AjPV1 genome consists of two dsRNA segments: dsRNA1 (2,360 bp) encoding RNA-dependent RNA polymerase (RdRp) and dsRNA2 (2,301 bp) encoding a capsid protein (CP).
  • Phylogenetic analysis confirmed AjPV1 as a new member of the family Partitiviridae, related to the genus Betapartitivirus.

Conclusions:

  • AjPV1 is the first mycovirus reported from Aplosporella javeedii.
  • The discovery of AjPV1 expands the known diversity of mycoviruses within the Partitiviridae family.
  • Further research is warranted to understand the biological role and impact of AjPV1 on its host fungus and plant disease development.

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