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Updated: Nov 18, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Ultrafast fluorescent probe with near-infrared analytical wavelength for fluoride ion detection in real samples
Lihong Li1, Min Zhang2, Lei Ding2
1Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, PR China; College of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, PR China; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, PR China.
Abstract:
The first ultrafast fluorescence probe with response time in seconds (10 s) for fluoride ions (F-) has been proposed by conjugating dimethylthiophosphoryl group as a recognition unit with the near-infrared fluorophore of hemicyanine. The response mechanism is the F--induced cleavage of the dimethylthiophosphoryl group, along with the liberation of the fluorophore, which results in a distinctly enhanced fluorescence intensity at 730 nm (λex = 680 nm). The fluorescence enhancement of the probe is directly proportional to the F- concentration in the range of 10-300 µM with the detection limit of 4.28 µM. The probe has been successfully used to determine F- concentration in real water and toothpaste samples as well as image F- in living cells. The simplicity and quick response of this probe endow it with the ability of detecting F- rapidly in real samples.
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