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Updated: Nov 18, 2025

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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Steroid receptor coactivator 3 (SRC-3/AIB1) is enriched and functional in mouse and human Tregs

Bryan C Nikolai1,2, Prashi Jain3,4, David L Cardenas3,4

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. nikolai@bcm.edu.

Scientific Reports
|February 10, 2021
PubMed

Insights

Regulatory T cells (Tregs) are crucial for preventing autoimmunity. This study reveals that the SRC-3 coactivator is essential for Treg function, highlighting its role in immune regulation and cancer evasion.

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Regulatory T cells (Tregs) are vital for maintaining self-tolerance and preventing autoimmune diseases.
  • The SRC-3 coactivator is implicated as an oncogene in various cancers and influences multiple transcription factors.
  • SRC-3 knockout mice exhibit lymphoproliferation and heightened inflammatory responses.

Purpose of the Study:

  • To investigate the role and enrichment of SRC-3 in regulatory T cells (Tregs).
  • To determine the impact of SRC-3 depletion or inhibition on Treg function and characteristics.

Main Methods:

  • Bioinformatic analysis of publicly available data.
  • Cellular assays involving Treg induction and suppression of T cell proliferation.
  • Pharmacological inhibition of SRC-3 activity.

Main Results:

  • SRC-3 is significantly enriched in both mouse and human Tregs.
  • Depletion or inhibition of SRC-3 impairs Treg phenotypic characteristics.
  • SRC-3 deficiency leads to a loss of Treg-mediated suppression of T cell proliferation.

Conclusions:

  • SRC-3 acts as a crucial coactivator in Tregs, actively contributing to the prevention of autoimmunity.
  • SRC-3's role in Treg biology may facilitate immune evasion strategies employed by cancers.

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