Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Characterization of Brucella polysaccharide B.

D R Bundle¹, J W Cherwonogrodzky, M B Perry

¹Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.

Infection and Immunity

|May 1, 1988

Summary

A new method for extracting Brucella polysaccharide B yielded a pure glucan, free from O polysaccharide contaminants. This pure Brucella polysaccharide B showed no serological reactivity, clarifying previous findings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Draft Genome Sequences of Bordetella hinzii and Bordetella trematum.

Genome announcements·2013

Same author

Structural characterization and serological specificities of lipopolysaccharides from Salmonella enterica serovar Gallinarum biovar Pullorum standard, intermediate and variant antigenic type strains.

Veterinary microbiology·2007

Same author

Ability of Escherichia coli O157:H7 isolates to colonize the intestinal tract of conventional adult CD1 mice is transient.

Canadian journal of microbiology·2004

Same author

Synthesis of di- to hexasaccharide 1,2-linked beta-mannopyranan oligomers, a terminal S-linked tetrasaccharide congener and the corresponding BSA glycoconjugates.

The Journal of organic chemistry·2001

Same author

Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459).

European journal of biochemistry·2001

Same author

Structural analysis of the lipopolysaccharide from the nontypable Haemophilus influenzae strain SB 33.

European journal of biochemistry·2001

Area of Science:

Microbiology
Biochemistry
Immunology

Background:

Brucella polysaccharide B extraction methods often yield O polysaccharide contaminants.
Previous serological studies of Brucella polysaccharide B may be affected by these impurities.

Purpose of the Study:

To develop a novel method for isolating pure Brucella polysaccharide B.
To characterize the structure of pure Brucella polysaccharide B.
To re-evaluate the serological reactivity of Brucella polysaccharide B.

Main Methods:

Mild leaching of Brucella cells for polysaccharide extraction.
High-performance liquid chromatography (HPLC) for separation.
Chemical composition analysis, methylation studies, and NMR spectroscopy for structural elucidation.

Main Results:

A new mild leaching protocol successfully isolated pure Brucella polysaccharide B, free from O polysaccharide.
Structural analysis revealed Brucella polysaccharide B as cyclic 1,2-linked beta-D-glucopyranosyl polymers (degree of polymerization 17-24).
Pure Brucella polysaccharide B exhibited no precipitation or inhibition in serological tests with Brucella anti-A/anti-M sera.

Conclusions:

The novel leaching method provides a pure Brucella polysaccharide B preparation.
The structural characteristics of Brucella polysaccharide B are elucidated.
Previous reported serological activities of Brucella polysaccharide B were attributed to O polysaccharide contaminants.

Related Experiment Videos