Related Experiment Video
Updated: Nov 18, 2025
Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
Published on: January 12, 2022
Correlations Between Retinal Optical Coherence Tomography and Histopathology in Preclinical Safety Assessment of
Annie Zimmerman1, Ryan F Boyd2, Krishna Yekkala1
1Department of Pathology, 537465Charles River Laboratories, Mattawan, MI, USA.
Methods:
Twelve studies that involved intravitreal or subretinal test article administration in multiple laboratory animal species and employed both OCT and histopathologic assessment were identified. These data were compared to determine the degree of correlation for each identified abnormality.
Results:
Severity of observed OCT and histopathological changes ranged from minimal to severe, with moderate and severe changes having a higher rate of agreement between the 2 techniques. Changes to well-defined structures, including the retinal blood vessels, optic nerve, and retinal pigment epithelium, also showed a strong correlation.
Conclusions:
There was a strong correlation between OCT and histopathology in both intravitreal and subretinal injection studies, demonstrating the value of collaboration between the study ophthalmologist and pathologist and offering translatable means to monitor pharmacological or toxicological effects in preclinical toxicological studies.
