Techniques for subretinal injections in animals.

Area of Science:

• Veterinary Ophthalmology
• Preclinical Research
• Ocular Surgery

Background:

• Subretinal injections are vital in animal models for evaluating gene and cell therapies.
• Veterinary ophthalmologists are key due to expertise in comparative ocular anatomy and non-human eye surgery.
• Successful subretinal injections require understanding species-specific approaches and potential complications.

Purpose of the Study:

• To summarize diverse subretinal injection methods across common animal models.
• To provide insights from literature and author experience for surgical success.
• To educate surgeons on performing subretinal injections in preclinical research.

Main Methods:

• Review of published literature on subretinal injection techniques in animal models.
• Compilation of author experience and expertise in veterinary ophthalmology.
• Focus on common laboratory animal species used in ocular research.

Main Results:

• Detailed descriptions of various subretinal injection approaches.
• Identification of potential complications associated with each technique.
• Guidance tailored to different animal species and their ocular structures.

Conclusions:

• Standardized knowledge of subretinal injection techniques is essential for reproducible research outcomes.
• This manuscript serves as a valuable resource for veterinary ophthalmologists and researchers.
• Effective subretinal injections contribute to the advancement of ocular therapies in preclinical studies.