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Updated: Nov 17, 2025
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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
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Elevated EXO1 expression is associated with breast carcinogenesis and poor prognosis
Jingjing Liu1,2,3,4,5, Jin Zhang1,2,3,4,5
13rd Department of Breast Cancer, China Tianjin Breast Cancer Prevention, Treatment and Research Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Annals of Translational Medicine
|February 11, 2021
Summary
Elevated Exonuclease 1 (EXO1) expression is linked to breast cancer development and poorer prognosis. High EXO1 levels may serve as a biomarker for breast cancer treatment strategies.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer is a leading cause of cancer mortality in women globally.
- Exonuclease 1 (EXO1) plays roles in DNA repair and recombination.
- The prognostic significance of EXO1 in breast cancer requires further investigation.
Purpose of the Study:
- To investigate the prognostic value of EXO1 in breast cancer.
- To explore the association between EXO1 expression and breast carcinogenesis.
Main Methods:
- Analysis of 1,215 breast cancer samples from The Cancer Genome Atlas (TCGA).
- Verification of EXO1 mRNA expression using RT-qPCR in breast cancer cell lines.
- Correlation analysis of EXO1 copy number with its expression.
- Kaplan-Meier analysis and gene expression profiling.
Main Results:
- Significantly increased EXO1 expression observed in breast cancer tissues.
- Elevated EXO1 mRNA levels confirmed in breast cancer cells (MDA-MB231) versus normal cells (MCF-10A).
- EXO1 copy number correlated with expression levels.
- High EXO1 expression associated with poor prognosis and altered cell cycle pathways.
Conclusions:
- Elevated EXO1 expression is linked to breast cancer development and poor prognosis.
- EXO1 may serve as a potential biomarker for breast cancer treatment.
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