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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

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Recent Advances in Quenchbody, a Fluorescent Immunosensor.

Jinhua Dong^1,2,3, Hiroshi Ueda^1,2

¹Tokyo Tech World Research Hub Initiative (WRHI), Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan.

Sensors (Basel, Switzerland)

|February 12, 2021

Summary

Quenchbodies (Q-bodies) are novel fluorescent immunosensors offering simple, sensitive antigen detection. This review details their principles, construction, and applications in diagnostics and monitoring.

Keywords:

antibody biomarker detection fluorescence immunoassay quench

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Immunotechnology

Background:

Immunodetection leverages specific antigen-antibody reactions for detecting various substances.
Traditional methods like LC-MS are complex and costly.
Immunodetection offers simplicity, selectivity, and broad applicability in diagnostics and monitoring.

Purpose of the Study:

To review the principle, construction, and applications of Quenchbodies (Q-bodies).
To highlight Q-bodies as a novel fluorescent immunosensor technology.
To discuss the current progress and potential of Q-body development.

Main Methods:

Discussion of the underlying principle of Q-body fluorescence.
Overview of Q-body construction and labeling techniques.
Compilation of diverse applications showcasing Q-body utility.

Main Results:

Q-bodies are antibody fragments labeled with fluorescent dyes.
Antigen binding to Q-bodies results in a measurable increase in fluorescence intensity.
This fluorescence change enables simple, sensitive, and operator-friendly antigen detection.

Conclusions:

Q-bodies represent a significant advancement in fluorescent immunosensors.
Their ease of use and high sensitivity make them ideal for various detection tasks.
Q-bodies show great promise for future applications in disease diagnosis and environmental monitoring.