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Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

947
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
947
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

662
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
662
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

772
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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Updated: Nov 17, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
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Estimating the Reduced Scattering Coefficient of Turbid Media Using Spatially Offset Raman Spectroscopy.

Sara Mosca1, Priyanka Dey2, Marzieh Salimi2

  • 1Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, UK Research and Innovation, Harwell Campus, Didcot OX11 0QX, United Kingdom.

Analytical Chemistry
|February 12, 2021
PubMed
Summary
This summary is machine-generated.

A new Spatially Offset Raman Spectroscopy (SORS) method estimates the reduced scattering coefficient (μs') in turbid samples. This technique enables more accurate photon propagation simulations for diverse scientific applications.

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Area of Science:

  • Photonics
  • Spectroscopy
  • Optical properties of materials

Background:

  • Spatially Offset Raman Spectroscopy (SORS) is used to analyze turbid samples.
  • Accurate knowledge of the reduced scattering coefficient (μs") is crucial for SORS.
  • Current methods for determining μs" can be limited.

Purpose of the Study:

  • To develop a novel method for estimating the reduced scattering coefficient (μs").
  • To validate the method using Spatially Offset Raman Spectroscopy (SORS).
  • To assess the accuracy of the proposed estimation technique.

Main Methods:

  • Utilized the variation of Raman signal with SORS spatial offset.
  • Assumed negligible absorption at laser and Raman wavelengths.
  • Employed a calibration procedure on a PTFE model sample.

Main Results:

  • Successfully estimated the μs" coefficient of various turbid samples.
  • Achieved a Root Mean Square Error of Prediction (RMSEP) below 18%.
  • Demonstrated the μs"-dependency of the Raman signal with spatial offset.

Conclusions:

  • The proposed SORS-based method provides a reliable estimation of μs".
  • Accurate μs" values facilitate improved numerical simulations in SORS.
  • This method has broad applicability in fields utilizing photon propagation in turbid media.