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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Complete Genome Sequence of Burkholderia cenocepacia Phage Mica

James Garcia1, Guichun Yao1, Maria Guadalupe Vizoso-Pinto2

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|February 12, 2021
PubMed

Insights

We identified Burkholderia phage Mica, a novel myophage targeting the multidrug-resistant pathogen Burkholderia cenocepacia. This discovery offers potential new avenues for combating infections in cystic fibrosis and chronic granulomatous disease patients.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • * Burkholderia cenocepacia is a multidrug-resistant Gram-negative bacterium.
  • * This pathogen frequently colonizes patients with chronic granulomatous disease and cystic fibrosis, leading to severe respiratory infections.
  • * Effective therapeutic options are limited due to high levels of antimicrobial resistance.

Purpose of the Study:

  • * To characterize a newly discovered bacteriophage, designated Burkholderia phage Mica.
  • * To investigate the potential of Burkholderia phage Mica as a therapeutic agent against Burkholderia cenocepacia infections.

Main Methods:

  • * Genomic analysis of Burkholderia phage Mica.
  • * Comparative analysis of phage structural proteins with known myophages.
  • * Prediction of the phage's life cycle (lysogenic or lytic).

Main Results:

  • * Burkholderia phage Mica was identified and characterized.
  • * Structural protein analysis suggests Mica is a myophage.
  • * Based on sequence similarity to Enterobacteria phage P2 and Burkholderia phage KL3, Mica is predicted to be a lysogenic myophage.

Conclusions:

  • * Burkholderia phage Mica represents a novel phage with potential for targeting Burkholderia cenocepacia.
  • * Its predicted lysogenic nature warrants further investigation for phage therapy applications.
  • * This finding contributes to the growing field of phage biocontrol for multidrug-resistant bacteria.

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