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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Mass Analyzers: Common Types01:19

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
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Hydrodynamic assisted multiparametric particle spectrometry.

Alberto Martín-Pérez1, Daniel Ramos2, Marina L Yubero1

  • 1Bionanomechanics Lab, Instituto de Micro y Nanotecnología, IMN-CNM (CSIC), Isaac Newton 8 (PTM), E-28760 Tres Cantos, Madrid, Spain.

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Summary

This study introduces a novel method for real-time particle analysis using nanomechanical resonators. The technique accurately measures mass density and cell properties, advancing cell biology research.

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Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
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Area of Science:

  • Biophysics
  • Nanotechnology
  • Cell Biology

Background:

  • Real-time analysis of single analytes in flow is crucial for cell biology.
  • Hydrodynamic focusing is a key technique for manipulating micro/nanoparticles.

Purpose of the Study:

  • To theoretically predict and experimentally demonstrate hydrodynamic focusing using hollow nanomechanical resonators.
  • To develop a method for unambiguous, real-time classification and mass density measurement of flowing particles.
  • To characterize MCF-7 human breast adenocarcinoma cells and explore cell cycle studies.

Main Methods:

  • Utilizing an interferometric system for optical probing of flowing particles.
  • Tracking the fundamental mechanical mode of hollow nanomechanical resonators.
  • Acquiring frequency shift, velocity, and reflectivity for particle characterization.

Main Results:

  • Accurate mass density measurements for PMMA (1.35 ± 0.07 g·mL⁻¹) and silica particles (1.7 ± 0.2 g·mL⁻¹).
  • Successful characterization of MCF-7 cells with a measured mass density of 1.11 ± 0.08 g·mL⁻¹.
  • Demonstrated high-throughput analysis (300 cells/minute) with observed size-dependent properties.

Conclusions:

  • The developed technique enables unambiguous, real-time classification of flowing particles based on hydrodynamic forces.
  • This method provides accurate mass density measurements, validated with standard particles.
  • The approach shows potential for individual cell cycle studies by analyzing cell size and density variations.