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Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-Based Fluorescence In Situ

Dehong Hu1, Yi Cui1,2, Lye M Markillie1

  • 1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2021
PubMed

Keywords:
Bacterial cellsGene expressionPhoto-blinkingSingle-molecule FISHSuper-resolution fluorescence imagingmRNA copies

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