Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.3K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.4K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Submolecular modulation of PIEZO1 mechanotransduction with wireless tailor-made nanoswitches.

Bioactive materials·2026
Same author

Nano-enabled enzyme-assisted layer-by-layer coating prevents biofilm formation on urinary catheters.

Acta biomaterialia·2026
Same author

Polyelectrolyte complexed alginate-oligochitosan nanoparticles for co-delivery of doxorubicin and indocyanine green in triple negative breast cancer therapy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Unilateral Proptosis Mimicking an Eye Tumour: A Case Report.

Cureus·2026
Same author

Multifunctional Hyaluronic Acid/Graphite Nanoplatelet Hydrogels as Tools for Spinal Cord Regeneration.

ACS applied materials & interfaces·2025
Same author

Surface-engineered gold nanorods for targeted delivery of PD-L1 siRNA and cancer chemo-phototherapy.

Nanoscale·2025
Same journal

Development of a device useful to reproducibly produce large quantities of viable and uniform stem cell spheroids with controlled diameters.

Materials science & engineering. C, Materials for biological applications·2022
Same journal

Osteogenic and anti-inflammatory potential of oligochitosan nanoparticles in treating osteomyelitis.

Materials science & engineering. C, Materials for biological applications·2022
Same journal

Enhancing the bioactivity of melt electrowritten PLLA scaffold by convenient, green, and effective hydrophilic surface modification.

Materials science & engineering. C, Materials for biological applications·2022
Same journal

Optimal structural and physical properties of aerogels for promoting robust neurite extension in vitro.

Materials science & engineering. C, Materials for biological applications·2022
Same journal

Effect of recombinant BMP-2 and erythropoietin on osteogenic properties of biomimetic PLA/PCL/HA and PHB/HA scaffolds in critical-size cranial defects model.

Materials science & engineering. C, Materials for biological applications·2022
Same journal

A loofah-inspired scaffold with enhanced mimicking mechanics and tumor cells distribution for in vitro tumor cell culture platform.

Materials science & engineering. C, Materials for biological applications·2022
See all related articles

Related Experiment Video

Updated: Nov 17, 2025

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.2K

Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model.

Vicente Domínguez-Arca1, Rui R Costa2, Ana M Carvalho2

  • 1Biophysics and Interfaces Group, Department of Applied Physics, Faculty of Physics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain; 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal.

Materials Science & Engineering. C, Materials for Biological Applications
|February 13, 2021
PubMed
Summary
This summary is machine-generated.

Researchers created a model system mimicking extracellular vesicles (EVs) using layer-by-layer films and liposomes. This model shows embedded liposomes are internalized more by cancer cells than surface ones, advancing EV research.

Keywords:
Cell communicationExtracellular matrixGlycosaminoglycansLayer-by-layerLiposomesSupported vesicle layers

More Related Videos

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.8K
Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

3.8K

Related Experiment Videos

Last Updated: Nov 17, 2025

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.2K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.8K
Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

3.8K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are crucial for intercellular communication, but their transfer mechanisms are not fully understood.
  • Understanding EV interactions with the extracellular matrix (ECM) and cells is vital for therapeutic applications.

Purpose of the Study:

  • To develop a simplified model system that mimics EVs and their interactions with cellular and extracellular environments.
  • To investigate the role of ECM-mimicking layers in modulating liposome-cell interactions.

Main Methods:

  • Fabrication of a layer-by-layer (LbL) film using poly-l-lysine (PLL) and hyaluronic acid (HA) to mimic the ECM.
  • Incorporation of liposomes (EV analogs) loaded with Nile Red (cargo model) into the LbL film.
  • Quartz crystal microbalance (QCM) for component integration monitoring and breast cancer cell (MDA-MB-231) incubation studies.

Main Results:

  • Successful fabrication of an LbL film with embedded, intact liposomes confirmed by QCM.
  • Nile Red exhibited a fast, first-order release from liposomes.
  • Embedded liposomes showed significantly higher internalization by MDA-MB-231 cells compared to surface-exposed liposomes.

Conclusions:

  • The developed LbL system effectively models EV-like particle interactions with cells and the ECM.
  • ECM-mimicking layers enhance, rather than hinder, the internalization of EV analogs by cancer cells.
  • This model provides a valuable platform for studying EV transfer phenomena and developing targeted drug delivery systems.