miR-3574 ameliorates intermittent hypoxia-induced cardiomyocyte injury through inhibiting Axin1
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Updated: Nov 17, 2025
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
Qingshi Chen1,2, Guofu Lin2, Yongfa Chen3
1Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Fujian Medical University, Fengze, Quanzhou 362000, China.
Objective:
miRNAs play critical roles in the regulation of many cardiovascular diseases. However, its role and potential mechanism in cardiac injury caused by obstructive sleep apnea (OSA) remain poorly elucidated. In the present study, we aimed to investigate the effects of miR-3574 on cardiomyocyte injury under intermittent hypoxia (IH).
Results:
We confirmed that IH inhibited cell viability, induced cell apoptosis and suppressed miR-3574 expression in the H9c2. miR-3574 overexpression could ameliorate the effects of IH on the cell viability and cell apoptosis in the H9c2. Axin1 was a target gene of miR-3574, and miR-3574 overexpression reduced the expression of Axin1. miR-3574 could inhibit the IH-induced cardiomyocyte injury via downregulating Axin1. However, Axin1 could partially reverse these effects of miR-3574.
Conclusion:
Our study first reveals that miR-3574 could alleviate IH-induced cardiomyocyte injury by targeting Axin1, which may function as a novel and promising therapy target for OSA-associated cardiovascular diseases.
Methods:
H9c2 were exposed to IH condition. CCK-8 assay was applied to determine cell viability of H9c2. qRT-PCR was conducted to measure the expression level of mRNA and miRNA. Western blot assay was then performed to detect the protein levels. Finally, we used dual-luciferase reporter assay identify the potential target of miR-3574.
Insights
MicroRNA-3574 (miR-3574) protects against heart cell injury caused by obstructive sleep apnea (OSA). Overexpressing miR-3574 reduces cell death by targeting Axin1, offering a potential therapy for OSA-related heart conditions.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Sleep Medicine
Background:
- MicroRNAs (miRNAs) are key regulators in cardiovascular diseases.
- The specific role of miRNAs in cardiac injury from obstructive sleep apnea (OSA) is not well understood.
Purpose of the Study:
- To investigate the impact of miR-3574 on cardiomyocyte injury induced by intermittent hypoxia (IH).
- To explore the underlying mechanisms of miR-3574 in OSA-related cardiac damage.
Main Methods:
- Cell viability was assessed using CCK-8 assays.
- Gene and miRNA expression levels were measured by qRT-PCR.
- Protein levels were analyzed via Western blot.
- Dual-luciferase reporter assays identified miR-3574 targets.
Main Results:
- Intermittent hypoxia (IH) reduced H9c2 cell viability and increased apoptosis, while suppressing miR-3574.
- Overexpression of miR-3574 protected H9c2 cells against IH-induced injury.
- Axin1 was identified as a direct target of miR-3574; miR-3574 overexpression downregulated Axin1.
- miR-3574 alleviated IH-induced cardiomyocyte injury by reducing Axin1 expression, though Axin1 partially reversed these effects.
Conclusions:
- miR-3574 mitigates intermittent hypoxia-induced cardiomyocyte injury by targeting Axin1.
- This finding highlights miR-3574 as a potential therapeutic target for cardiovascular complications associated with obstructive sleep apnea.
