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Updated: Nov 17, 2025
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
Published on: July 13, 2013
Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes
Dhenesh Puvanendran1, Hager Souabni1, Dimitri Salvador2,3
1Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS UMR 7099, Institut de Biologie Physico-Chimique (IBPC), Université Paris Diderot, Sorbonne Paris Cité, PSL Research University, Paris, France.
Abstract:
Proteoliposome reconstitution is a method of choice for the investigation of membrane proteins as it allows their manipulation in the desired hydrophobic environment and allows one to tackle their study from both functional and structural points of view. Methods for their rapid and efficient reconstitution have been known for a long time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine for the obtention of monodisperse populations of proteoliposomes as well as for the quantitation of protein per liposome.
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