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Pluripotent stem cells in the research for extraembryonic cell differentiation
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Development, Growth & Differentiation
|February 14, 2021
Summary
Mouse embryonic stem cells (mESCs) and human pluripotent stem cells (hPSCs) are valuable tools for studying extraembryonic tissue differentiation. These stem cell models allow in vitro investigation of trophectoderm and primitive endoderm development, aiding embryonic research.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Genetics
Background:
- Mouse embryonic stem cells (mESCs) originate from the preimplantation embryo.
- mESCs are used to model differentiation of somatic and germ cells.
- mESCs also model extraembryonic tissues like trophectoderm (TE) and primitive endoderm (PrE).
Purpose of the Study:
- To investigate the utility of mESC cultures for studying TE and PrE differentiation.
- To explore the potential of human pluripotent stem cells (hPSCs) in modeling human extraembryonic lineage development.
Main Methods:
- Utilizing mESC cultures to induce and study TE and PrE differentiation in vitro.
- Employing hPSCs, including human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs), for extraembryonic lineage generation.
Main Results:
- mESC cultures effectively reproduce cellular and molecular events of differentiation.
- In vitro differentiation of TE and PrE from mESCs provides insights into developmental processes.
- hPSCs demonstrate capability in generating human extraembryonic lineages.
Conclusions:
- mESC cultures are appropriate systems for studying extraembryonic tissue differentiation when used correctly.
- hPSCs offer promising avenues for studying human extraembryonic lineage development in vitro.
Keywords:
embryonic stem cellepiblastinduced pluripotent stem cellpluripotent stem cellprimitive endodermtrophectodermMore Related Videos
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