Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

702
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
702
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

264
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
264
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

721
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
721
Diversity of Archaea IV01:29

Diversity of Archaea IV

257
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
257
Factors Influencing Microbial Growth: pH01:29

Factors Influencing Microbial Growth: pH

704
Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
704
Diversity of Archaea I01:30

Diversity of Archaea I

304
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
304

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of novel self-activating protein FfGH79 that regulates both mycelial growth and fruiting body development in Flammulina filiformis.

International journal of biological macromolecules·2026
Same author

NIR/pH-responsive arginine-ε-polylysine/black phosphorus nanocomposites for synergistic therapy of bacterial infections.

Materials today. Bio·2025
Same author

Widely Targeted Metabolomic and Network Pharmacology Analyses of Active Compounds Enriched from Ethanolic Extract of <i>Oudemansiella raphanipes</i>.

Foods (Basel, Switzerland)·2025
Same author

On-demand celastrol delivery by hyaluronic acid-porphyrinic metal-organic frameworks for synergistic sonodynamic/pharmacological antibacterial therapy.

International journal of biological macromolecules·2025
Same author

Genome-Wide Identification and Expression Analysis of the Cys2His2 Zinc Finger Protein Gene Family in <i>Flammulina filiformis</i>.

Journal of fungi (Basel, Switzerland)·2024
Same author

Morphology of Four Strains of Phellinoid Agaricomycetes and Microstructural and Physiological Properties of Their Exudates.

International journal of medicinal mushrooms·2024

Related Experiment Video

Updated: Nov 17, 2025

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

4.0K

Temperature affects substrate-associated bacterial composition during Ganoderma lucidum hyphal growth.

Bo Zhang1,1, Jie Zhou1,1, Xiaolin Li1,1

  • 1Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.

Canadian Journal of Microbiology
|February 16, 2021
PubMed
Summary

Temperature significantly impacts the bacterial diversity in Ganoderma lucidum substrates. Elevated temperatures alter microbial communities, affecting fungal growth and facilitating cultivation.

Keywords:
Ganoderma lucidumbacterial compositioncomposition bactérienneeffet de la températurehigh-throughput sequencingséquençage à haut débittemperature effect

More Related Videos

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
08:11

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

1.1K
Simulating Temperature in a Soil Incubation Experiment
08:39

Simulating Temperature in a Soil Incubation Experiment

Published on: October 28, 2022

3.3K

Related Experiment Videos

Last Updated: Nov 17, 2025

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

4.0K
Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
08:11

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

1.1K
Simulating Temperature in a Soil Incubation Experiment
08:39

Simulating Temperature in a Soil Incubation Experiment

Published on: October 28, 2022

3.3K

Area of Science:

  • Mycology and Microbiology
  • Fungal Biotechnology
  • Environmental Science

Background:

  • Ganoderma lucidum cultivation is influenced by substrate temperature.
  • Temperature affects the microbial community structure associated with G. lucidum.

Purpose of the Study:

  • To analyze bacterial diversity in G. lucidum substrate at varying temperatures.
  • To understand the impact of temperature on microbial communities and G. lucidum growth.

Main Methods:

  • Next-generation sequencing technology was employed to analyze bacterial diversity.
  • 15 substrate samples were analyzed across different temperature conditions.
  • Bioinformatic tools were used for sequence assignment and functional prediction (KEGG pathways).

Main Results:

  • Proteobacteria and Firmicutes dominated the bacterial phyla, showing opposite trends with increasing temperature.
  • Specific genera like Ochrobactrum and Rhodococcus were enriched at 40°C.
  • Bacterial genes involved in membrane transport were most abundant at 40°C.

Conclusions:

  • Temperature is a critical factor influencing G. lucidum mycelial morphology and substrate bacterial community.
  • Findings provide insights for optimizing G. lucidum cultivation through temperature control.