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Mapping multiple endocrine disrupting activities in Virginia rivers using effect-based assays.

Diana A Stavreva1, Michael Collins2, Andrew McGowan1

  • 1Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

The Science of the Total Environment
|February 16, 2021
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Summary

Endocrine disrupting chemical (EDC) activity was detected in over 70% of tested Virginia river water samples using novel fluorescent bioassays. These findings highlight widespread contamination impacting ecosystems and potentially human health.

Keywords:
Biological activityEndocrine disrupting compoundsHormonesRiver water

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Area of Science:

  • Environmental Chemistry
  • Toxicology
  • Molecular Biology

Background:

  • Water sources face contamination from endocrine disrupting chemicals (EDCs) that are not routinely monitored.
  • Phenotypic bioassays offer a novel method for detecting and quantifying EDCs.
  • Developing sensitive assays is crucial for environmental risk assessment.

Purpose of the Study:

  • To develop and apply novel fluorescent cell-based bioassays for detecting EDC activity in river water.
  • To screen water samples from Virginia rivers for interactions with key nuclear receptors.

Main Methods:

  • Developed cell lines with fluorescent chimeric constructs to detect interactions with androgen (AR), glucocorticoid (GR), aryl hydrocarbon (AhR), and thyroid receptors (TRβ).
  • Concentrated water samples (200×) collected from 45 sites across two major Virginia rivers in summers 2016-2018.
  • Screened samples for receptor activation indicating the presence of specific EDCs.

Main Results:

  • Over 70% of sites showed AR activity, and 60% showed AhR activity.
  • Significant GR (22%) and TRβ (42%) activation was detected in tested water samples.
  • Multiple sites exhibited activity for more than one receptor, indicating complex EDC mixtures.

Conclusions:

  • Virginia river water is widely contaminated with substances exhibiting endocrine-disrupting activities.
  • The presence of complex EDC mixtures poses risks to aquatic ecosystems and potentially human health.
  • Phenotypic bioassays are effective tools for monitoring environmental contaminants.