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Gold Nanoparticle Synthesis
Published on: July 10, 2021
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Universal gold nanoparticle modified hybrid monolithic substrate developed for facile in-column
Ling-Yu Zhao1, Yue-Lun Sun1, Li Mao2
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing, 210023, China.
Talanta
|February 17, 2021
Summary
Researchers developed a universal hybrid monolithic column substrate for easy post-functionalization. This gold nanoparticle-modified material simplifies sample preparation for biomedical and environmental analyses, saving time and effort.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Organic-inorganic hybrid monolithic columns are valuable for sample pretreatment in complex analyses.
- Optimizing preparation methods for diverse analytes and functional groups is time-consuming.
Purpose of the Study:
- To develop a versatile hybrid monolithic column for flexible post-functionalization.
- To create a universal substrate using gold nanoparticles (GNPs) as an intermediary bridge.
Main Methods:
- Synthesized single- and bi-functionalized hybrid monolithic columns.
- Immobilized gold nanoparticles (GNPs) onto the monoliths.
- Functionalized columns with thioglycolic acid (TGA) and an anti-thrombin aptamer.
- Evaluated GNP adsorption capacities and functionalization densities.
Main Results:
- Compared GNP adsorption capacities across five different hybrid monoliths.
- Achieved high coverage densities for TGA (168.41 nmol μL⁻¹) and aptamer (2205.8 pmol μL⁻¹).
- Demonstrated excellent adsorption selectivity for thrombin using the aptamer-functionalized column in solid-phase microextraction.
Conclusions:
- The GNPs-modified amine- and thiol-bi-functionalized hybrid monolithic column serves as an effective universal substrate.
- This approach facilitates easy and efficient post-modification of separation materials.
- It significantly reduces the time and labor associated with optimizing preparation processes for various analytes.
