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Related Experiment Videos

Pre-analytical variation in alanine aminotransferase.

S G Ruby1, N E Reiber, R E Lonser

  • 1Department of Pathology, Hinsdale Hospital, IL 60521.

Clinical Chemistry
|April 1, 1988
PubMed
Summary

Alanine aminotransferase (ALT) activity in blood specimens remains stable for up to 72 hours, regardless of storage temperature (4°C or 22°C). This finding is crucial for understanding pre-analytical variations in ALT testing.

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Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Laboratory Medicine

Background:

  • Pre-analytical variables significantly impact laboratory test results.
  • Alanine aminotransferase (ALT) is a key biomarker for liver function.
  • Understanding specimen stability is vital for accurate diagnostic testing.

Purpose of the Study:

  • To investigate the impact of pre-analytical temperature variations on ALT activity.
  • To assess the stability of ALT in both serum and whole blood specimens.
  • To establish reliable timeframes for ALT sample analysis.

Main Methods:

  • Serum and whole blood samples with normal ALT activity were stored at 4°C and 22°C.
  • Aliquots were assayed at various time points up to 72 hours.
  • Statistical analysis using Analysis of Variance (ANOVA) was performed.

Main Results:

  • No statistically significant changes in ALT activity were observed in serum samples.
  • No statistically significant changes in ALT activity were observed in whole blood samples.
  • ALT stability was confirmed for both refrigerated and room temperature storage up to 72 hours.

Conclusions:

  • Pre-analytical storage temperature (4°C or 22°C) does not significantly affect ALT activity for up to 72 hours.
  • This suggests a wide window for sample collection and analysis without compromising ALT results.
  • These findings support current laboratory practices for ALT specimen handling.

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