Related Experiment Video
Updated: Nov 17, 2025

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
Published on: July 16, 2013
Centrosomes: Til O-GlcNAc Do Us Apart
Aiyun Yuan1, Xiangyan Tang1, Jing Li1
1Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, China.
Abstract:
The centrosome apparatus is vital for spindle assembly and chromosome segregation during mitotic divisions. Its replication, disjunction and separation have to be fine-tuned in space and time. A multitude of post-translational modifications (PTMs) have been implicated in centrosome modulation, including phosphorylation, ubiquitination and acetylation. Among them is the emerging O-linked N-acetylglucosamine (O-GlcNAc) modification. This quintessential PTM has a sole writer, O-GlcNAc transferase (OGT), and the only eraser, O-GlcNAcase (OGA). O-GlcNAc couples glucose metabolism with signal transduction and forms a yin-yang relationship with phosphorylation. Evidence from proteomic studies as well as single protein investigations has pinpointed a role of O-GlcNAc in centrosome number and separation, centriole number and distribution, as well as the cilia machinery emanating from the centrosomes. Herein we review our current understanding of the sweet modification embedded in centrosome dynamics and speculate that more molecular details will be unveiled in the future.
More Related Videos
07:06Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
Published on: June 23, 2023
09:39Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
Related Concept Videos
Centrioles and Centrosomes
Near the end of the prophase, also called late prophase or...
Histone Variants at the Centromere
Centrosome Duplication
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Attachment of Sister Chromatids
Protein Glycosylation
Glycosylation occurs in...