Tooth shade variation in Indian population: An objective guide to age estimation
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Updated: Nov 17, 2025

Oral Health Assessment by Lay Personnel for Older Adults
Published on: February 2, 2020
N Srikant1, Annapurna S Intern2, G Vaishnavi2
1Center for Forensic Odontology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Introduction:
The color of the teeth is affected by chronological age due to the variations of the hard and soft tissue structure of the teeth. There are very few studies have assessed the shade of the Enamel and correlated it with the age changes. Hence the study aimed to assess the enamel shade with the aging in the individual.
Materials And Methods:
The central incisors of 388 individuals (5-78 years) were assessed for tooth color using a VITA 3D master shade guide and the Hue, Value and Chroma were noted. Mean grey values of the teeth were obtained by image manipulation using Image J software and normalized using the values obtained from the 18% contrast grey card. Chi square tests and linear regression analysis is used to find associations with age and tooth shade variations.Results: The teeth had higher score of Hue, Value and Chroma in older individuals. The tooth shade tended to shift towards redder hue with older age group. Simple linear regression analysis showed a significant correlation of age with normalized grey value in association with the shade parameters. (r = 0.717, SEE = 12.322 years)Conclusion: Tooth color changes with age and the mean age in grey values and shade guides can be a useful tool for age estimation.
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