Related Experiment Video
Updated: Nov 17, 2025
Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
Published on: January 19, 2015
Biochemical detection of capsid in the nucleus during HIV-1 infection
Anastasia Selyutina1, Felipe Diaz-Griffero1
1Department of Microbiology and Immunology, Einstein, Bronx, NY 10461, USA.
Abstract:
To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic markers to assess the bona fide origin of the fractions. This protocol can be applied in both cycling and non-cycling human cells. For complete details on the use and execution of this protocol, please refer to Selyutina et al. (2020a).
