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Related Experiment Video

Updated: Nov 16, 2025

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

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Intelligent Multidimensional Purity Analysis and Confirmation Tool for Multiple Attribute Analysis.

Jee Seong Kwak^1,2, Wenyao Peter Zhang², Debasis Mallik^1,2

¹Flow Chemistry Facility, Centre for Catalysis Research and Innovation (CCRI) and Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.

Analytical Chemistry

|February 19, 2021

Summary

Developing chiral active pharmaceutical ingredients (APIs) requires optimizing multiple quality attributes simultaneously. A new multidimensional liquid chromatography tool enables parallel measurement of these attributes from a single injection, accelerating drug discovery.

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Area of Science:

Analytical Chemistry
Pharmaceutical Sciences
Organic Chemistry

Background:

Chiral active pharmaceutical ingredients (APIs) offer enhanced specificity for chiral biological targets compared to achiral compounds.
Stereoselective synthesis of chiral APIs demands parallel optimization of critical quality attributes, including chemical and stereochemical purity, from early drug development stages.

Purpose of the Study:

To introduce a novel multidimensional liquid chromatographic tool designed for simultaneous measurement of multiple quality attributes.
To facilitate the parallel optimization of asymmetric synthesis for chiral APIs.
To expedite the identification of on-analysis artifacts, thereby accelerating drug development.

Main Methods:

Development of a multidimensional liquid chromatography system utilizing a unique heart-cut valve.
Recirculation of chromatographic eluent through one or more stationary phases for iterative analyte measurement.
Simultaneous assessment of multiple quality attributes from a single analytical injection.

Main Results:

The chromatographic tool enables iterative measurement of a target analyte from a single injection.
This approach aids in distinguishing between impurities formed during reaction and those arising during analysis.
The system is particularly effective in identifying on-analysis artifacts, a common bottleneck in drug development.

Conclusions:

The reported multidimensional liquid chromatography tool significantly enhances the efficiency of chiral API development.
Simultaneous measurement of critical quality attributes streamlines the optimization of asymmetric synthesis.
Accelerated identification of analytical artifacts reduces resource expenditure and speeds up the drug development timeline.