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Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos
Yuichiro Mishima1, Kunio Inoue2
1Department of Frontier Life Sciences, Faculty of Lifesciences, Kyoto Sangyo University, Kyoto, Japan. mishima@cc.kyoto-su.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2021
Abstract:
Many proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.
