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The theory and practice of ultrafast liquid chromatography: A tutorial
M Farooq Wahab1, Daipayan Roy1, Daniel W Armstrong1
1Department of Chemistry & Biochemistry, University of Texas at Arlington, TX, USA.
Analytica Chimica Acta
|February 20, 2021
Summary
Ultrafast chromatography enables separations in under a second using advanced instrumentation and short columns. Signal processing techniques are crucial for resolving overlapping peaks in these rapid chromatographic methods.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- High-throughput research demands faster analytical methods.
- Advances in instrumentation enable sub-second chromatographic separations.
Purpose of the Study:
- To review the theoretical principles and practical solutions for ultrafast chromatography.
- To examine instrumentation and address challenges in rapid separations.
Main Methods:
- Demonstration of rapid separations in packed beds, open tubular columns, and monoliths.
- Examination of instrumentation for short columns (0.5–5 cm).
- Application of signal processing techniques (Fourier transform deconvolution, power laws, derivatives, curve fitting) to improve resolution.
Main Results:
- Feasibility of sub-minute to sub-second chromatography demonstrated.
- Challenges like peak overlap addressed using statistical overlap theory.
- Example of ten peaks separated in under one second presented.
Conclusions:
- Ultrafast chromatography is a viable emerging field for rapid analytical separations.
- Advanced instrumentation and signal processing are key to overcoming challenges like peak overlap.
- Further exploration of ultrafast techniques in SFC and CE is warranted.
Keywords:
Chiral separationsHigh-throughput liquid chromatographyHydrophilic interaction liquid chromatographyNarrow open-tubular columnsShort columnsSignal processingSub-second separationsMore Related Videos
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