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A Xanthene Dye-based Sensor for Viscosity and Cell Imaging.

Bo Yu1,2, Ying Zhou3, Lihua Dou1,2

  • 1College of Resources and Environmental Engineering, Mianyang Normal University, Mianyang, 621000, Sichuan, China.

Journal of Fluorescence
|February 20, 2021
PubMed
Summary
This summary is machine-generated.

A novel xanthene dye, ImX, exhibits viscosity-selective fluorescence, enhancing emission with increasing solvent viscosity. This dye shows low cytotoxicity and can track cellular viscosity changes, aiding in biological imaging.

Keywords:
Fluorescence imagingViscosityXanthene

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Area of Science:

  • Organic Chemistry
  • Materials Science
  • Biophysical Chemistry

Background:

  • Xanthene dyes are widely used fluorescent probes.
  • Developing novel probes with specific environmental responses is crucial for advanced imaging.
  • Understanding cellular microenvironment viscosity is important for biological studies.

Purpose of the Study:

  • To synthesize and characterize a new xanthene dye, ImX.
  • To investigate the photophysical properties of ImX, particularly its response to solvent viscosity and pH.
  • To evaluate the potential of ImX as a fluorescent tracer for cell imaging, specifically for detecting viscosity changes.

Main Methods:

  • Facile synthesis of ImX via reaction of 4-(1H-Imidazol-1-yl)benzaldehyde with N, N-diethyl-3-aminophenol, followed by treatment with p-chloranil.
  • Spectroscopic analysis to determine absorption and emission maxima in water.
  • Fluorescence spectroscopy to study the viscosity-selective response in solvents of varying viscosities (1.1 cp to 1248 cp) and across a pH range (2.5 to 10.0).
  • Cytotoxicity assessment and cell imaging experiments to detect Monensin-triggered viscosity enhancement.

Main Results:

  • ImX was successfully synthesized.
  • ImX exhibits maximum absorption at 562 nm and emission at 583 nm in water.
  • The dye demonstrates a significant viscosity-selective fluorescent response with emission enhancement as viscosity increases.
  • The viscosity-selective fluorescence is maintained over a broad pH range (2.5–10.0).
  • ImX shows low cytotoxicity and is effective in cell imaging for detecting viscosity changes.

Conclusions:

  • ImX is a novel xanthene dye with excellent viscosity-sensitive fluorescent properties.
  • Its broad pH compatibility and low cytotoxicity make it a promising fluorescent probe for biological applications.
  • ImX can be utilized as a tracer for monitoring intracellular viscosity changes, offering a new tool for cell imaging and biological research.