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Updated: Nov 16, 2025
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Published on: November 1, 2024
Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines
C H Cole Sims1, Matias I Autio2, Adriana Buskin1
1Translational and Clinical Research Institute, Newcastle University, UK.
None:
Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.
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