Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SIGNAL: A Scalable, Real-World Model for Rapid Intraoperative Molecular Classification of Gliomas Using Stimulated Raman Histology.

medRxiv : the preprint server for health sciences·2026

Same author

CNS-Obsidian: A Neurosurgical Vision-Language Model Built From Scientific Publications.

Neurosurgery·2026

Same author

AI-Powered Pipeline Transforms Neurosurgical Articles Into High-Quality Graphical Abstracts.

Neurosurgery practice·2026

Same author

Intelligent histology for tumor neurosurgery.

Neuro-oncology advances·2026

Same author

Anatomic Predilection of Isocitrate Dehydrogenase-Mutant Gliomas: A Multi-Institutional Spatial Analysis.

Neurosurgery·2026

Same author

Natural Language Processing Methods Automate Molecular Marker Extraction From Glioma Pathology Reports.

Neurosurgery·2026

Same journal

Intraoperative technological advances and new frontiers in precision glioma surgery.

Journal of neuro-oncology·2026

Same journal

Optimizing the Stupp protocol for treatment of glioblastoma: eliminating age bias, enhancing treatment timing, use of stereotactically-guided sequential boost, and dexamethasone dosing.

Journal of neuro-oncology·2026

Same journal

Gamma knife radiosurgery for renal cell carcinoma brain metastases across systemic therapy eras: survival, intracranial failure, and lesion-level predictors.

Journal of neuro-oncology·2026

Same journal

Real-time confocal imaging for evaluation of dose-dependent effects of gamma knife radiosurgery on U87 glioblastoma cell line.

Journal of neuro-oncology·2026

Same journal

Clinical, surgical, and molecular characteristics and outcomes of adult patients with diffuse midline glioma, H3 K27-altered, treated with surgical resection.

Journal of neuro-oncology·2026

Same journal

Intratumoral serotonin and antidepressants in glioblastoma patients: narrowing the uncertainties.

Journal of neuro-oncology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 16, 2025

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Label-free brain tumor imaging using Raman-based methods.

Todd Hollon¹, Daniel A Orringer²

¹University of Michigan, Ann Arbor, USA.

Journal of Neuro-Oncology

|February 21, 2021

Summary

This summary is machine-generated.

Label-free Raman imaging techniques, including Raman spectroscopy, CARS microscopy, and stimulated Raman histology (SRH), offer real-time chemical and histologic data for brain tumor management. These methods detect microscopic tumor infiltration and aid in intraoperative diagnosis.

Keywords:

Coherent Raman imaging Coherent anti-Stokes Raman scattering microscopy Intraoperative pathology Label-free imaging Molecular imaging Raman spectroscopy Stimulated Raman histology Stimulated Raman scattering microscopy

More Related Videos

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

Related Experiment Videos

Last Updated: Nov 16, 2025

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

Area of Science:

Neurosurgical Oncology
Biomedical Optics
Chemical Imaging

Background:

Label-free Raman-based imaging provides intrinsic biochemical contrast for tissue analysis.
Enables optical sectioning and detection of microscopic tumor infiltration.
Facilitates intraoperative diagnosis of brain tumor subtypes.

Purpose of the Study:

To review the application of three Raman-based imaging methods in neurosurgical oncology.
Highlighting Raman spectroscopy, CARS microscopy, and stimulated Raman histology (SRH).
To demonstrate their potential in improving brain tumor patient management.

Main Methods:

Raman spectroscopy for ex vivo and in vivo tissue chemical characterization.
Coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering microscopy for submicron resolution imaging.
Intraoperative SRH with AI-assisted interpretation.

Main Results:

Raman spectroscopy differentiates normal and tumor-infiltrated tissues by macromolecule content.
CARS and SRH detect microscopic brain tumor infiltration in fresh specimens.
Raman-based methods show potential for intraoperative glioma molecular classification.

Conclusions:

Label-free Raman imaging enhances brain tumor patient management.
Improves detection of tumor infiltration and guides surgical resection.
Provides intraoperative histopathologic and molecular diagnostic capabilities.