SARS-CoV-2 Genomes From Oklahoma, United States

Area of Science:

• Virology
• Genomics
• Molecular Biology

Background:

• Genomic sequencing is crucial for understanding SARS-CoV-2 pathogenicity and tracking viral evolution.
• Regular sequencing is essential for monitoring mutations and genomic modifications in different geographical locations during the pandemic.

Purpose of the Study:

• To sequence SARS-CoV-2 from clinical samples in Oklahoma to identify mutations and genomic modifications.
• To analyze the phylogenetic relationships and potential structural impacts of identified mutations.

Main Methods:

• Whole-genome sequencing of five SARS-CoV-2 clinical samples from Oklahoma.
• Identification of previously reported and potential novel mutations in key genes (S, ORF1ab, N, ORF7b, ORF8).
• Phylogenetic analysis and structural characterization of identified mutations.

Main Results:

• Sequencing identified previously reported mutations (e.g., D614G, P4715L, S194L, R203K, G204R).
• Potential novel mutations were detected in S (G1167V), ORF1ab (A6269S, P3371S), ORF7b (T28I), and ORF8 (G96R) genes.
• Phylogenetic analysis showed similarity to global SARS-CoV-2 strains; mutations may affect spike protein flexibility and nucleocapsid structure.

Conclusions:

• The study provides valuable genomic data on SARS-CoV-2 circulating in Oklahoma.
• Identified mutations, including potential novel ones, may influence viral pathogenicity and evolution.
• Further research is needed to fully elucidate the functional and clinical significance of these genomic alterations.