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Related Experiment Video

Updated: Nov 16, 2025

Chromosome Preparation From Cultured Cells

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

Chromosome analysis and sorting.

Jaroslav Doležel¹, Sergio Lucretti², István Molnár¹

¹Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|February 22, 2021

Summary

This summary is machine-generated.

Flow sorting of plant chromosomes aids genome assembly and gene cloning. Careful sample preparation is crucial for high-purity chromosome isolation using flow cytometry.

Keywords:

DNA amplification DNA isolation cell cycle synchronization gene mapping and cloning genome sequencing liquid chromosome suspension marker development mitotic metaphase chromosomes repetitive DNA labelling

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Published on: February 6, 2012

Area of Science:

Plant genetics
Cytogenetics
Genomics

Background:

Flow cytometric analysis and sorting of plant mitotic chromosomes is a specialized technique.
It is essential for advancing plant genetics, genome assembly, and gene cloning.
Dissecting complex plant genomes via flow sorting simplifies DNA analysis.

Purpose of the Study:

To review the applications of plant chromosome sorting.
To detail sample preparation, analysis, and sorting methods for high-purity fractions.
To discuss the suitability of flow-sorted chromosomes for downstream applications.

Main Methods:

Utilizing commercially available electrostatic droplet sorters for chromosome analysis and sorting.
Developing protocols for cell cycle synchrony, metaphase arrest, and intact chromosome release.
Employing fluorescent staining and DNA repeat labeling for chromosome discrimination.

Main Results:

High resolution and stability in flow sorters are key for resolving and purifying chromosomes.
Adequate sample preparation, focusing on intact mitotic metaphase chromosomes, is the primary challenge.
Optimized protocols yield high-purity flow-sorted chromosome fractions.

Conclusions:

Flow sorting is a powerful tool for plant genome research, despite technical challenges.
Mastery of sample preparation is critical for successful chromosome isolation and purity.
Flow-sorted chromosomes are valuable resources for various molecular and genomic techniques.