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Metal-responsive reversible binding of triplex-forming oligonucleotides with 5-hydroxyuracil nucleobases
Kotaro Nishiyama1, Keita Mori1, Yusuke Takezawa1
1Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shionoya@chem.s.u-tokyo.ac.jp.
Abstract:
Metal-responsive triplex-forming oligonucleotides (TFOs) were synthesised by incorporating 5-hydroxyuracil (UOH) nucleobases as metal recognition sites. Binding of the UOH-containing TFO to the target natural DNA duplexes was reversibly regulated by the addition and removal of GdIII ions under isothermal conditions.
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