Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coupling Single Molecules to DNA-Based Optical Antennas with Position and Orientation Control.

ACS photonics·2026
Same author

The Chromatin-Modifying Protein RCOR2/CoREST2 Safeguards Axon-Dendrite Growth and Microtubule Stability in Brain Neurons.

Journal of neurochemistry·2026
Same author

Distinct nanoscale membrane organizations of mucins and <i>trans</i>-sialidases in <i>Trypanosoma cruzi</i>.

bioRxiv : the preprint server for biology·2026
Same author

Modulation of Single-Molecule Emission at Hexagonal Boron Nitride Surfaces.

Nano letters·2026
Same author

Three-dimensional plasmonic nanopores for DNA-PAINT and dual-material Au/Si architectures.

Journal of nanobiotechnology·2026
Same author

Distance-Dependent Energy Transfer Between Organic Fluorophores and Single-Walled Carbon Nanotubes.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Nov 16, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.3K

Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET.

Alan M Szalai1, Bruno Siarry1, Jerónimo Lukin2

  • 1Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad Autónoma de Buenos Aires, Argentina.

Nano Letters
|February 23, 2021
PubMed
Summary

Super-resolution Förster resonance energy transfer (FRET) imaging, called STED-FRET, now offers detailed molecular interaction insights. This advanced technique overcomes previous limitations, enabling precise visualization of biomolecular events in complex biological systems.

Keywords:
bioimagingenergy transferfluorescencesingle-moleculesuperresolution microscopy

More Related Videos

F&#246;rster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
07:09

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Published on: March 16, 2022

2.8K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.1K

Related Experiment Videos

Last Updated: Nov 16, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.3K
F&#246;rster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
07:09

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Published on: March 16, 2022

2.8K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.1K

Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Biology

Background:

  • Förster resonance energy transfer (FRET) imaging reveals molecular interactions but is limited by diffraction-limited resolution, providing averaged data.
  • Coupling super-resolution microscopy with FRET has been a significant technical challenge in biological imaging.

Purpose of the Study:

  • To develop and present STED-FRET, a novel method for achieving super-resolved energy transfer imaging.
  • To enhance the accuracy of quantifying molecular interactions and suppress signals from non-interacting molecules.

Main Methods:

  • Implementation of Stimulated Emission Depletion (STED) microscopy combined with FRET principles.
  • Validation using DNA-origami nanostructures and uniformly double-labeled microtubules.
  • Application to image biomolecular interactions within the neuronal membrane-associated periodic skeleton (MPS).

Main Results:

  • STED-FRET successfully achieved super-resolution imaging of energy transfer events.
  • The method demonstrated higher spatial resolution compared to conventional FRET imaging.
  • Accurate quantification of molecular interactions was achieved, with improved suppression of non-interacting partner signals.

Conclusions:

  • STED-FRET is a broadly applicable method for super-resolved FRET imaging.
  • This technique provides more precise insights into biomolecular interactions at the nanoscale.
  • STED-FRET advances the study of molecular dynamics in cellular structures like the neuronal MPS.