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Updated: Nov 16, 2025

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
Published on: October 9, 2021
Synthesis of redox-active fluorinated 5-hydroxytryptophans as molecular reporters for biological electron transfer
Amanda Ohler1, Hanna Long, Kei Ohgo
1Department of Chemistry, East Carolina University, Greenville, NC, USA. offenbachera17@ecu.edu.
Abstract:
Fluorinated 5-hydroxytryptophans (Fn-5HOWs) were synthesized in gram scale quantities and incorporated into a β-hairpin peptide and the protein azurin. The redox-active Fn-5HOWs exhibit unique radical spectroscopic signatures that expand the function of as probes for biological electron transfer.
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