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Identification of microalgae cultured in Bold's Basal medium from freshwater samples, from a high-rise city
Charmaine Lloyd1,2, Kai Heng Tan3, Kar Leong Lim3
1School of Life Sciences and Chemical Technology - Microalgal Research Group, Ngee Ann Polytechnic, Clementi, Singapore. calloyd@swin.edu.au.
Abstract:
This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, EH = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.
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