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Updated: Nov 16, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Mitochondrial genome of Diachrysia nadeja (Lepidoptera: Noctuoidea: Noctuidae) and phylogenetic analysis
Shanshan Gao1,2, Shuang Xue1,2, Yuanchen Zhang1,2
1College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, China.
Abstract:
Diachrysia nadeja is a polyphagous herbivorous moth within the family Noctuidae. In this study, we sequenced and analyzed the complete mitochondrial genome (mitogenome) of D. nadeja. This mitogenome was 15,242 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and 2 ribosomal RNA unit genes (rRNAs). Gene order was conserved and identical to most other previously sequenced Noctuidae. Except for cox1 started with CGA, all other PCGs started with the standard ATN codons. Most of the PCGs terminated with the stop codon TAA, whereas cox1, cox2, and nad4 end with the incomplete codon T-. The whole mitogenome exhibited heavy AT nucleotide bias (80.5%). Phylogenetic analysis showed that D. nadeja got together with three Ctenoplusia species (C. agnata, C. limbirena, and C. albostriata) with high support value, indicating Diachrysia had a closer relationship with Ctenoplusia within Noctuidae.
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