Tenacibaculum pelagium sp. nov., isolated from marine sediment

A novel bright yellow pigmented, Gram-stain-negative, gliding, aerobic and rod-shaped marine bacterium, designated strain S7007^T, was isolated from a marine sediment sample taken from Jingzi Wharf, Weihai, China. The bacterium was able to grow at 4-33 °C (optimum 28 °C), at pH 6.5-9.0 (optimum 7.0) and with 2.0-4.0% (w/v) NaCl (optimum 3.0%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain S7007^T was associated with the genus Tenacibaculum and showed highest similarity to Tenacibaculum adriaticum JCM 14633^T (98.0%). The average nucleotide identity (ANI) scores of strain S7007^T with T. adriaticum JCM 14633^T and T. maritimum NBRC 110778^T were 78.3% and 77.1%, respectively and the Genome-to-Genome Distance Calculator (dDDH) scores were 20.5% and 19.9%, respectively. The sole isoprenoid quinone was MK-6 and the major cellular fatty acids (> 10.0%) were iso-C_15:0, iso-C_15:0 3-OH, iso-C_{15: 1} G and summed feature 3 (comprising C_16:1 ω7c and/or C_16:1 ω6c). The major polar lipids of strain S7007^T were phosphatidylethanolamine, phosphatidyldimethylethanolamine, one unidentified lipid and two unidentified aminolipids. The genomic DNA G + C content was 30.9 mol %. The combined phenotypic data and phylogenetic inference that strain S7007^T should be classified as a novel species in the genus Tenacibaculum, for which the name Tenacibaculum pelagium sp. nov. is proposed. The type strain is S7007^T (= MCCC 1H00428^T = KCTC 72941^T).