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Updated: Jun 11, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

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Published on: July 27, 2018

Coding-Complete Genome Sequence of a Partitivirus Isolated from Pine Processionary Moth Eggs.

Franck Dorkeld¹, Réjane Streiff¹, Carole Kerdelhué¹

¹CBGP, INRAE CIRAD IRD Institut Agro Université de Montpellier, Montpellier, France.

Microbiology Resource Announcements

|February 26, 2021

Summary

Two novel partitivirus sequences were identified in the pine processionary moth, *Thaumetopoea pityocampa*. These findings reveal potential RNA-dependent RNA polymerase and coat protein genes within the Lepidoptera species.

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Published on: July 27, 2018

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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

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Published on: August 31, 2022

Area of Science:

Virology
Entomology
Molecular Biology

Background:

Partitiviruses are non-enveloped viruses with a bisegmented double-stranded RNA genome.
The family *Partitiviridae* encompasses several genera of viruses infecting fungi, plants, and insects.
Lepidoptera, including the pine processionary moth (*Thaumetopoea pityocampa*), are significant insect pests with complex ecological roles.

Purpose of the Study:

To identify and characterize novel viral sequences within transcriptomic data from *Thaumetopoea pityocampa* eggs.
To determine the genetic makeup and potential functions of discovered viral segments.

Main Methods:

Analysis of transcriptomic data sets from *Thaumetopoea pityocampa* eggs.
Identification of coding-complete nucleotide sequences.
Bioinformatic prediction of open reading frames and encoded proteins.

Main Results:

Discovery of two coding-complete nucleotide sequences belonging to a partitivirus (family *Partitiviridae*).
Each viral segment was found to encode a single open reading frame.
Predicted proteins include an RNA-dependent RNA polymerase and a coat protein.

Conclusions:

The study reports the first identification of partitivirus sequences in *Thaumetopoea pityocampa*.
These findings expand the known host range of partitiviruses within the Lepidoptera order.
The identified sequences provide insights into the molecular biology of insect-associated viruses.