WIPI1 promotes osteosarcoma cell proliferation by inhibiting CDKN1A

Related Experiment Video

Updated: Nov 16, 2025

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.5K

Tianfei Ran1, Song Ke1, Xin Song1

  • 1Orthopeadic Dept. Xinqiao Hospital, Army Medical University, Chongqing, China.

Gene
|February 26, 2021
PubMed

Insights

WIPI1 is highly expressed in osteosarcoma and promotes cancer cell proliferation. This study investigated WIPI1

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • WIPI1 (WD repeat domain, phosphoinositide interacting 1) is highly expressed in osteosarcoma.
  • Understanding WIPI1's role is crucial for osteosarcoma research.

Purpose of the Study:

  • To investigate the mechanisms by which WIPI1 influences osteosarcoma cell proliferation.
  • To identify key molecular pathways regulated by WIPI1 in osteosarcoma.

Main Methods:

  • Affymetrix microarray analysis to identify differentially expressed genes.
  • RT-PCR and Western-blot for gene expression verification.
  • Flow cytometry, MTT assays, cell counts, and nude mouse tumorigenicity assays to assess proliferation.

Main Results:

  • WIPI1 is significantly upregulated in osteosarcoma.
  • WIPI1 regulates cell cycle-related genes including CDKN1A, CDK4, and CCND1.
  • WIPI1 overexpression promotes osteosarcoma cell proliferation and tumor growth in vivo.

Conclusions:

  • WIPI1 promotes osteosarcoma cell proliferation.
  • WIPI1 may exert its oncogenic effects by modulating cell cycle gene expression.
  • Targeting WIPI1 could be a potential therapeutic strategy for osteosarcoma.
Keywords:
CDKN1ACell cycleCell proliferationOsteosarcomaWIPI1

More Related Videos

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.9K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.4K

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.3K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
37.4K